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Perspectives on Lipase Enzyme Technology
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Authors: J. Geraldine Sandana Mala (Golden Jubilee Biotech Park for Women Society, Chennai, India); Satoru Takeuchi (Factory of Takeuchi Nenshi, Takenen, Ishikawa, Japan) 
Book Description:
Lipases are triacylglycerol acylhydrolases that catalyse the hydrolysis of triglycerides to release concomitant free fatty acids and glycerol. Lipases are versatile enzymes with multiplexity of catalysis and therefore, constitute an important class of industrial enzymes of recent interests in basic and applied research. This book serves as a handbook for researchers in the Life Sciences and particularly for those who plan to investigate microbial lipases. It is also aimed to provide an updated and comprehensive review of laboratory activities on lipases, in particular from the microbial sources, thereupon, to impart good scientific perceptions to the reader in this discipline. The production characteristics are fundamental for manipulation of maximal enzyme titres. Considerable importance is also necessitated to gain genetically modified strains to enable enormous availability of the recombinant lipases for structural elucidations. Detailed descriptions on the isolation methods, production requirements, protein investigations and molecular cloning of the microbial lipases are presented. Bioinformatics approaches are also specifically discussed. Furthermore, lipase as a biocatalyst candidate is specifically included to target the application prospects of the lipase. Experimental protocols are presented for bench work practice. An appendix section is supplemented with significant data for the researcher as a reference tool for their routine laboratory investigations.

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Table of Contents:


Chapter 1. Biotechnology: the science and origin of lipase, pp. 1-11

Chapter 2. Isolation strategies of microbial lipases, pp. 13-20

Chapter 3. Lipase production-defining the media, pp. 21-28

Chapter 4. Lipase purification and characterization, pp. 29-49

Chapter 5. Lipase structure, pp. 51-60

Chapter 6. Molecular cloning and overexpression, pp. 61-85

Chapter 7. Bioinformatics, pp. 87-93

Chapter 8. Lipases: biocatalysts for the future, pp. 95-102

Section I:
Experiments, pp. 103-111

Section II:
Appendix, pp. 113-128

Section III:
Abbreviations, pp. 129-133
Section IV:
Bibliography, pp. 135-137

      Biotechnology in Agriculture, Industry and Medicine
   Binding: Hardcover
   Pub. Date: 2009
   Pages: 147 pp.
   ISBN: 978-1-60741-977-8
   Status: AV
Status Code Description
AN Announcing
FM Formatting
PP Page Proofs
FP Final Production
EP Editorial Production
PR At Prepress
AP At Press
AV Available
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Perspectives on Lipase Enzyme Technology