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Quantitative Immunohistology: Problems and Solutions
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Authors: Anthony S-Y Leong (University of Newcastle, Australia and others), Trishe Y-M Leong (Victorian Cytology Service, Melbourne, Australia) 
Book Description:
As immunohistochemistry enters its fourth decade as a diagnostic tool, this analytical procedure continues to experience poor reproducibility, albeit in specific situations. The problem becomes more important with the mounting pressure to employ the assay in a quantitative manner for the assessment of therapeutic and prognostic markers in a number of neoplasms. Qualitative applications of immunohistochemistry are well established and continue to increase with the range of sensitive antibodies and detection systems available, however, numerous variables that influence the immunoexpression of proteins in formalin-fixed tissue continue to exist in the pre-analytical and analytical phases of the test procedure. This book emphasizes these problems in detail and suggests possible solutions.

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Table of Contents:

Chapter 1. Introduction, pp. 1-2

Chapter 2. The Immunohistological Assay- A Total Test Approach, pp. 3-4

Chapter 3. Pre-Analytical Variables: Tissue Preservation, Transportation, Fixation and Tissue Processing, pp. 5-12

Chapter 4. Analytical Variables- Antigen Retrieval, Antibodies, Detection Systems and Controls, pp. 13-32

Chapter 5. Post-Analytical Phase, pp. 33-37

Chapter 6. Receptor Detection for Targeted Therapies, pp. 39-44

Chapter 7. Interobserver Variability in Quantitative Immunohistology, pp. 35

Chapter 8. Conclusion, pp. 37-49


      Immunology and Immune System Disorders
   Binding: Softcover
   Pub. Date: 2010
   Pages: 6 x 9
   ISBN: 978-1-61668-261-3
   Status: AV
Status Code Description
AN Announcing
FM Formatting
PP Page Proofs
FP Final Production
EP Editorial Production
PR At Prepress
AP At Press
AV Available
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Quantitative Immunohistology: Problems and Solutions