Nova Publishers
My Account Nova Publishers Shopping Cart
HomeBooksSeriesJournalsReference CollectionseBooksInformationSalesImprintsFor Authors
  Top » Catalog » Books » Education » Book Chapters » Focus on Leukemia Research Chapters » My Account  |  Cart Contents  |  Checkout   
Quick Find
Use keywords to find the product you are looking for.
Advanced Search
What's New? more
Theory of Literature
Shopping Cart more
0 items
Shipping & Returns
Privacy Notice
Conditions of Use
Contact Us
Notifications more
NotificationsNotify me of updates to Paraffin Section Immunotyping of Leukaemias (pp. 95-111)
Tell A Friend
Tell someone you know about this product.
Paraffin Section Immunotyping of Leukaemias (pp. 95-111) $25.00
Authors:  Juneja, S. (Royal Melbourne Hospital, Melbourne, Australia) Westerman, D. and Seymour, J. F. (Peter MacCallum Cancer Center, Melbourne, Australia)
To better understand underlying biological heterogeneity and optimize management, leukaemias are increasingly being characterized by multiple methodologies. In addition to morphology, cytogenetics and molecular studies, immunophenotyping is important for both the accurate diagnosis and the detection of residual disease. Immunophenotyping can be performed by flow cytometry (FCM) in most cases and is the preferred method whenever a fresh tissue samples is available; however, there are circumstances where the leukaemias need to be characterised immunophenotypically in paraffin tissue sections (paraffin section immunophenotyping PSI). The circumstances in which PSI is useful include, dry or blood tap of the bone marrow, difficulty in establishing lineage by FCM and detection of residual disease post-chemotherapy not identified by FCM; it is also applicable where leukaemia was not suspected in the differential diagnosis and appropriate samples for FCM were not taken. PSI also has the advantage with good preservation of cellular morphology, which can be visualised, in tissue sections unlike FCM. PSI is applicable in tissues fixed in formalin, Bouin's or B5 fixatives. The most commonly used method is the labelled streptavidin-biotin peroxidase method with diaminobenzidine or aminoethyl carbozole used as a chromogen. The panel of antibodies utilized varies with the subtype of leukaemia under investigation. In the context of an acute leukaemia it generally includes non-lineage specific markers CD34 and TdT and lineage specific markers including myeloperoxidase (granulocytic), CD3 (T lymphoid) and CD79a (B lymphoid). In chronic leukaemias depending upon whether the cell of origin is B, T or NK lineage appropriate combination of antibodies can be used. In conclusion PSI has a definite role in the diagnosis, characterization and monitoring of residual disease in selected cases of leukaemia. Its role continues to expand as the available antibody panel grows the technique is automated and becomes more widely available. 

Available Options:
Special Focus Titles
01.Violent Communication and Bullying in Early Childhood Education
02.Cultural Considerations in Intervention with Women and Children Exposed to Intimate Partner Violence
03.Chronic Disease and Disability: The Pediatric Lung
04.Fruit and Vegetable Consumption and Health: New Research
05.Fire and the Sword: Understanding the Impact and Challenge of Organized Islamism. Volume 2

Nova Science Publishers
© Copyright 2004 - 2021

Paraffin Section Immunotyping of Leukaemias (pp. 95-111)