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Distribution of Tumor Necrosis Factor Producing Cells in Chronic Tonsillitis $100.00
Authors:  Milan Stankovic, Miroljub Todorovic, Verica Avramovic, Misa Vlahovic and Dragan Mihailovic
Abstract:
Objective is to determine and quantify the production of TNF- in chronic tonsillitis.
Material and Methods
The study comprised of 23 patients with chronic tonsillitis, divided in two groups: 10 patients with tonsillar hypertrophy (TH) with average age 9.0 2.7 years, and 13 patients with recurrent tonsillitis (RT) aged 23.1 5.2 years.
Highly sensitive labeled streptavidin-biotin horse reddish peroxidase immunohistochemical method (LSAB+/HRP) was used for detection of TNF-producing cells. Quantification of TNF-was made for crypt epithelium, germinative centers, roundness of follicles, interfollicular areas and subepithelial area. Quantification of lymph follicles and germinative centers included: areal (mm2), median optical density (au), circumference (mm), Ferret diameter (mm), and integrated optical density (IOD).Objective is to determine and quantify the production of TNF- in chronic tonsillitis.
Material and Methods
The study comprised of 23 patients with chronic tonsillitis, divided in two groups: 10 patients with tonsillar hypertrophy (TH) with average age 9.0 2.7 years, and 13 patients with recurrent tonsillitis (RT) aged 23.1 5.2 years.
Highly sensitive labeled streptavidin-biotin horse reddish peroxidase immunohistochemical method (LSAB+/HRP) was used for detection of TNF-producing cells. Quantification of TNF-was made for crypt epithelium, germinative centers, roundness of follicles, interfollicular areas and subepithelial area. Quantification of lymph follicles and germinative centers included: areal (mm2), median optical density (au), circumference (mm), Ferret diameter (mm), and integrated optical density (IOD). 


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Distribution of Tumor Necrosis Factor Producing Cells in Chronic Tonsillitis