DEVELOPMENT OF A DIAGNOSTIC SYSTEM FOR OSTEOARTHRITIS USING A PHOTOACOUSTIC MEASUREMENT METHOD AND TIME-RESOLVED AUTO-FLUORESCENCE, pp. 179-190
Authors: Masato Sato, Miya Ishihara, Genya Mitani, Toshiharu Kutsuna, Jeong Ik Lee, Makoto Kikuchi, Joji Mochida, Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Kanagawa, Japan, and others
Abstract: We have developed new methods to measure some essential properties of cartilage: a photoacoustic measurement method and time-resolved fluorescence spectroscopy. These can be used to evaluate the outcomes of tissue-engineered cartilage, regenerated articular cartilage tissue after surgery, and the degenerated cartilage of osteoarthritis patients. A nanosecond-pulsed laser, which is completely noninvasive, is focused onto the target cartilage and induces a photoacoustic wave, which will propagate with attenuation, which is affected by the viscoelasticity of the surrounding cartilage. The decay time during which the amplitude of the photoacoustic wave is reduced by a factor of 1/e is the key numerical value used to characterize and evaluate the viscoelasticity and rheological behavior of the cartilage. In this study, we also investigated whether pulsed laser irradiation and the measurement of excited autofluorescence allow us to noninvasively evaluate tissue characters in real time. Our findings show that time-resolved laserinduced fluorescence spectroscopy is useful for evaluating tissue-engineered cartilage. This measurement system, predicated on the interactions between optics and living organs, is a suitable methodology for diagnosis during arthroscopy, because it allows the quantitative and multidirectional evaluation of the original function of the cartilage based on a variety of parameters.
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