SERPINA5 Expression in the Male Reproductive Tract is altered with Advanced Age pp.211-218
Authors: (Matthew D Anway—Center For Reproductive Biology, Department of Biological Sciences, University of Idaho, Moscow, ID)
Abstract: The serine proteinase inhibitor SERPIN5A is abundantly expressed in the male reproductive tract in rodents and humans. SERPIN5A is critical for maintaining proper development of sperm. In mouse model, SERPIN5A knockout males are infertile. SERPIN5A is responsible for inactivating serine proteinases, such as protein c, urokinase and tissue plasminogen activator. SERPIN5A has been associated with several disease states including infertility, thrombosis and prostate disease in humans and rodents. The expression and localization of SERPIN5A is well documented in the early development of the male reproductive tract. However, the mRNA expression pattern of SERPIN5A in the aging male reproductive tract is unclear. The Brown Norway rat develops reproductive tract abnormalities with advanced age. To this end, we investigated the transcript level of SERPIN5A in the young (4 month), mid age (10 month) and aged (19 month) male reproductive tract. SERPIN5A was differentially expressed in the male reproductive tract with advanced age. The gene expression of SERPIN5A was up-regulated in the Sertoli cells, dorsal and ventral prostate tissue with advanced age. The epididymis had the largest increase between the young and the aged tissue. SERPIN5A was down-regulated in the lateral prostate tissue. SERPIN5A had the highest expression in the seminal vesicles, but was not differentially regulated with age. The expression of SERPIN5A is sensitive to androgens and although testosterone levels are lower with advanced age it is not responsible for the observed alterations in the gene expression. We hypothesize that the changes in SERPIN5A expression in the male reproductive tract with advance age in the Brown Norway rat is the result of the disease progression.