The Participation of Terbium Ion in Blood Coagulation pp. 217-230
Authors: (L.S. Nikolaeva, A.N. Semenov, A.E. Homutov, Department of Chemistry Lomonosov Moscow State University, and others)
Abstract: In this chapter our viewpoint on the problem of Tb3+ ion participation in blood coagulation process is considered on the base of our new investigations of complex formation Tb3+ ion with heparin as continuation of earlier published work, biological experiments in vivowith the blood plasma of the laboratory rats and the modern enzymatic theory of blood clotting. In this chapter the investigations of the Terbium properties by the synthesis of the solid terbium heparinat Tbhep, the execution of elements, thermogravimetric and IR-spectrum analysis of this solid complex and by the biological experiments in vivo with the laboratory rats are presented. The injections of the terbium heparinat and terbium chloride were made in the peritonium of the laboratory rats. The conclusions are the following: 1) The solid terbium heparinat in the concentration that is equivalent to 50 IU heparin is a coagulant because it forms the complex Tbhep2 5- which inactivates the anticoagulant activity of the endogenous heparin. The solid terbium heparinat is not anticoagulant on two reasons: 1) concentration of Terbium ion is near zero because of small dissociation constant K* (log K*= −7,51), 2) complex CaTbHep is not formed. 2) Terbium chloride in the concentration of 1.5*10-5–1.5*10-2 is a coagulant because it forces Ca2+ ions out from complexes of Ca2+ ions with endogenous legands-anticoagulants:heparin, Citrat, Adenosinetriphosphat, Lactat, Glutamat and others. 3) In the blood clotting process the result of the competetive complexing of Tb3+ and Ca2+ ions withpolarheads of moleculesphosphatidyserine on the surface of cell membranes and two COO - groups ofY-carboxyglutamic acid that is the part of the structure of blood clotting factors does not exceed the stable coagulation effectTb3+ ions in the concentration of 1.52*10-5– 1.52*10-2.