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| Stationary Phase In Mycobacterium smegmatis: Stringently Influencing The Cell Surface, The Proteome, The Regulome and The Genome pp. 1-102 |
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Authors: (Abhinav Dey, Dipankar Chatterji, Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India)
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Abstract:
The Mycobacterium smegmatis strain mc2155 has proven to be a workhorse to comprehend the physiological landscape of the stationary phase and the molecular events that allow this specie to survive prolonged periods of starvation.
The hallmark of the stringent response is the accumulation of guanosine tetra- [ppGpp] and pentaphosphates [pppGpp], which probably bind RNA polymerase to regulate gene expression at certain promoters [Chatterji and Ojha, 2001]. So far, the rel gene in Gram positive organisms is clearly known to be responsible for the synthesis and hydrolysis of [p]ppGpp. However, the two activities are performed by two different proteins in Gram negative bacteria. During nutritional deprivation, M. smegmatis shows a stringent response, as characterized by the accumulation of [p]ppGpp, resulting in the repression of stable RNA species, such as rRNA and tRNA, with a concomitant change in colony morphology [Ojha et al,2000; Jain et al, 2006]. The analysis of the stationary-phase-induced changes in the lipid profile of cell surface biologically active lipid, glycopeptidolipid [GPL] of M. smegmatis revealed the biosynthesis of a polar GPL in [Ojha et al. 2002], whose structure was thereafter found to contain two units of a 3,4-di-O-methyl derivative of rhamnose attached to L-alaninol via a 1→2 linkage [Mukherjee et al, 2005] |
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