Contribution of CD40-Activated Naïve B Lymphocytes in the Modulation of CD27+ Memory B Cell Growth and Differentiation pp. 143-167
Authors: (Sonia Néron, Geneviève Côté, Nellie Dumont, Annie Roy, Jessie F. Fecteau, Marie-Ève McNeil, Héma-Québec, Ingénierie Cellulaire, Recherche et Développement, Québec, Québec, Canada, and others)
Abstract: Interactions between CD40+ B lymphocytes and CD154+ T lymphocytes are central in the development of humoral memory immune response. Binding strength and occupancy of CD40 receptors modulate proliferation and differentiation and by doing so, influences the balance between naïve and memory B lymphocyte subsets. We previously observed that memory B cell response to CD40-CD154 interaction was different given that naïve lymphocytes were present or not. As a result, we investigated whether interactions between naïve and memory B lymphocytes could participate in feedback mechanisms following high and low levels of CD40-CD154 stimulation. Noteworthy, emergence of CD70+ cells, largely among the naïve B lymphocytes, was major following a high level of CD40 stimulation. In these conditions, antibodies interfering with CD27-CD70 interaction or addition of exogenous CD70 molecules changed the balance between B lymphocyte subsets and regulate Ig secretion. Furthermore, the growth of IgG+ B lymphocytes was postponed when submitted to high CD40-CD154 stimulation in the presence of increased proportion of CD70+ B lymphocytes. Overall, our results show that B lymphocyte sub-populations can collaborate in vitro and suggest that such interactions could also participate in the regulation of germinal center reaction.
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