In Vitro Propagation of Olive Olea Europaea L. pp. 205-219
Authors: (E.B. Trabelsi, M. Msallem, Z. Belfeleh, N. Elloumi, R. Ghezel, S. Bouzid, Laboratory of Plant Biology and Biotechnology, Faculty of Sciences of Tunis, Tunisia, and others)
Abstract: In the present study we report an efficient protocol for high frequency plantlet regeneration from nodal explants of olive Olea europaea L. Nodal explants obtained from mature trees were grown on OM medium with different concentrations and combinations of cytokinin i.e. isopentenyladenine; 2i-P and auxin i.e. NAA to assess the optimum conditions for multiple shoots regeneration. The highest number of shoot buds was obtained with media containing 4 mgl-1 2-iP and 0.1 mgl-1 NAA. Shoot multiplication was maintained by repeated
subculturing of the developed shoots onto fresh media containing 2 mgl-1 zeatin in combination with IBA at 0.1 mgl-1. Elongated shoots were rooted on half strength MS medium supplemented with 1 mgl-1 NAA. The addition of activated charcoal to the rooting medium did not improve the rooting rates recorded. It did, however, positively affect the root quality of regenerated plantlets. The well-rooted plantlets were hardened and transplanted to a glasshouse and soil with 90% successful plantlet survival.