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Macromolecular Synthesis in Mitochondria in the Adrenal Glands of Aging Mice as Observed by Electron Microscopic Radioautography pp. 71-174 $100.00
Authors:  (Tetsuji Nagata, Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan, and others)
Abstract:
For the purpose of studying the aging changes of macromolecular synthesis in animal cells by means of microscopic radioautography, we studied many groups of aging mice during development and aging from fetal day 19 to postnatal newborn, juvenile at day 1, 3, 7, 14, young adults at month 1, 2, 6, and senescent adults up to month 12 and 24 (2 years). They were injected with 3H-thymidine, 3H-uridine or 3H-leucine, precursors for DNA, RNA and proteins, respectively, sacrificed and the adrenal tissues were taken out, fixed and processed for light and electron microscopic radioautography. On many electron microscopic radioautograms the localization of silver grains demonstrating DNA, RNA and proteins in adrenal cortical and medullary cells in respective aging groups were analyzed qualitatively. The number of silver grains and the number of cell organelles in each cell of each animal in respective aging groups were analyzed quantitatively in relation to the aging of individual animals. The results revealed that the localization of respective precursors as well as the number of silver grains in cell nuclei, cell organelles, changed due to the aging of animals. The numbers of labeled nuclei and cell organelles, as well as the numbers of silver grains in nuclei and cell organelles changed due to aging of individual animals. The number of mitochondria, the number of labeled mitochondria and the mitochondrial labeling index labeled with silver grains were counted in each cell in 3 zones of the adrenal cortices, the glomerulosa, the fasciculate and the reticularis as well as in the medullary cells. It was demonstrated that the numbers of mitochondria, the numbers of labeled mitochondria and the labeling indices showing DNA, RNA and protein synthesis at various ages from embryonic day 19 to postnatal newborn day 1, 3, 7, 14, adult month 1, 2 and 6, reaching the maxima, then decreased to senile year 1 to 2, showing the aging changes. The results indicated that mitochondria in adrenal cells synthesized nucleic acids and proteins independently from the nuclei, but their synthetic activities were affected from the aging of the individual animals. Based upon our findings, available literature on macromolecular synthesis in nuclei and mitochondria of various cells are also reviewed. 


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Macromolecular Synthesis in Mitochondria in the Adrenal Glands of Aging Mice as Observed by Electron Microscopic Radioautography pp. 71-174