DNA Microarray Analysis on the Murine Models for Endometriosis and Spontaneous Abortion pp. 347-362
Authors: (Ken Kusakabe, Ai Takeshita, Hideaki Abe, Tomohiro Kondo, Toshiya Okada, Department of Laboratory Animal Science, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Izumisano, Osaka, Japan, and others)
Abstract: DNA microarray is a powerful method for understanding the genetic basis of important traits by identifying underlying candidates from known gene databases or novel genes that influence trait expression. To clarify our understanding of disease causation we attempted to identify significant genetic alteration involved in disease etiology and development using mouse models for endometriosis and spontaneous abortion. DNA microarray analysis revealed changes in placental gene expression associated with spontaneous abortion, especially a complement activator ―adipsin‖ which was greatly up-regulated. Up-regulation of adipsin in aborted placentas was confirmed at the protein level, and suggested that adipsin and its complement system may have a crucial role in reproductive physiology and the failure of pregnancy. From the endometriosis models, chemokines and prostaglandin-related factors were found to be up-regulated. The pattern of expression of CXCL10, calbindin D-28K, prostaglandin E receptor 3 and prostaglandin I2 synthase in the mouse endometriosis model was similar to that in human endometriotic tissue.
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