Cellular Reprogramming- Advances and Opportunities for Applications in Veterinary and Animal Sciences pp. 195-214
Authors: (B. Singh, S.K. Gautam, M.S. Chauhan, S.K. Singla, S. Kumar, V. Kumar, P.S. Yadav, Indian Veterinary Research Institute, Palampur, India, and others)
Abstract: Nuclear reprogramming an intriguing phenomenon in which specialized somatic cells are induced to acquire pluripotency through global resetting of epigenetic modifications. The recent methods of inducing reprogramming through ectopic expression of specific transcription factors could enable the researchers bypass the use of nuclear transfer cloning, the commonly used method of reprogramming adult cells, which rely on availability of mammalian oocytes. The reprogrammed cells offer an attractive platform for researchers to elaborate the fundamental mechanisms of mammalian developmental biology. Recent breakthroughs in stem cell technology, especially the direct derivation of pluripotent cells from differentiated cells offer vast therapeutic implications in pathophysiology, regenerative medicine, in vitro disease modeling and pharmaceutical screening etc. The technology has enormous opportunities in livestock assisted reproduction, genetic engineering, and tissue generation for use in animal injuries for which natural healing does not deliver functional recovery, and for which current therapeutic strategies have minimal effectiveness.