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Shiitake Mushroom Submerged Culture and Morphogenesis: Regularities, Computations, Practical Use pp. 1-26 $100.00
Authors:  (Olga M. Tsivileva, Alexei N. Pankratov, Valentina E. Nikitina, Laboratory of Microbiology, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia, and others)
Abstract:
The advantage of submerged mushroom culture for the production of mushroom derivatives is clear: mycelia formed by growing pure strains in submerged culture under controlled conditions not only have a consistent composition, but are also safer. One of the first macrofungi to be cultivated at large scale in submerged culture was the basidiomycete Lentinula edodes (Berk.) Pegler, otherwise known as shiitake.
The topics on submerged cultivation related to biosynthesis of extracellular polysaccharides and other medicinal substances are covered. Attention is paid to the extracellular proteins production (oxidative enzymes, ribonucleases, enzymatic activity in dual cultures of Lentinula) and morphogenesis in liquid culture.
Then we turn our attention to the results that our group has obtained working with L. edodes over the last ten years.
The formation of pigmented mycelial film in submerged culture was assayed to find correlations with both the medium composition and the age of inoculums. The quantum chemical methods and QSAR were applied to test our supposition that a differential character of interaction between the amino acids under study and Ca2+, Mn2+ cations should be related not to the distinct electron structures of zwitter ions, but most likely to their differing hydrophobicity. 


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Shiitake Mushroom Submerged Culture and Morphogenesis: Regularities, Computations, Practical Use pp. 1-26