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NotificationsNotify me of updates to Insight into Force Transmission Along Actin Filaments: Sliding Movement of Actin Filaments Containing Inactive Components on Myosin Molecules pp. 257-269
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Insight into Force Transmission Along Actin Filaments: Sliding Movement of Actin Filaments Containing Inactive Components on Myosin Molecules pp. 257-269 $100.00
Authors:  (Syunsuke Matsushita, Kuniyuki Hatori, Department of Biosystem Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Japan)
Abstract:
The sliding movement of actin filaments, consisting of heterogeneous components,
on skeletal muscle myosin molecules was examined to specifically evaluate the effect of
internal modulation of the actin filaments for force transmission on the sliding
movement. Inactive actin molecules were prepared by conjugation with indocarbocyanine
fluorescent dyes (IC3-OSu or Cy3-NHS) in molar ratios greater than a 3-fold excess.
IC3-OSu is an analogue of Cy3-NHS, and it can bind to primary amino groups. IC3-
conjugated actin (IC3-actin) monomers were polymerized into the filaments which led to
complete impairment of both motile activity and myosin-ATPase activation. Filaments of
Cy3-conjugated actin (Cy3-actin) exhibited a decrease in velocity to a third of the value
(33%) observed for intact actin filaments. In the absence of ATP, dissociation rates of
IC3- and Cy3-actin filaments from myosin molecules were greater than those of intact
actin filaments, indicating that IC3- and Cy3-actin act as smaller resistance components
against sliding movement compared to intact filaments. Subsequently, two types of
copolymer filaments were prepared. The first type of copolymer were filaments
copolymerized homogeneously with intact actin monomers and IC3-actin monomers,
while the second kind were block copolymer filaments composed of two short filaments
of intact actin and IC3-actin. The sliding velocities of these copolymer filaments
hyperbolically decreased as the fraction of IC3-actin monomer increased. In practice,
75% IC3-actin within homogeneous copolymer was required to reduce the velocity by
half. In the case of block copolymer 65% IC3-actin led to the same decrease in velocity.
For Cy3-actin copolymer filaments similar differences between homogeneous and block
copolymer filaments were also observed. Drag ratio between IC3-actin (or Cy3-actin) and
intact actin was estimated by consideration of the force balance between the power force
and the drag force imposed on a filament during steady movement. Consequently, the
drag ratios of IC3-actin to intact actin were 0.31 (homogeneous copolymer) and 0.47
(block copolymer), respectively. Thus, IC3-actin incorporated homogeneous copolymers
exhibits a smaller resistance to sliding movement than IC3-actin modified block
copolymers. 


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Insight into Force Transmission Along Actin Filaments: Sliding Movement of Actin Filaments Containing Inactive Components on Myosin Molecules pp. 257-269