Significance of CD142-Positive Cell-Derived Microparticles after Percutaneous Coronary Intervention in Patients with Coronary Artery Disease (pp.261-270)
Authors: (Norihito Inami, Takayuki Kajiura, Kohichi Yamada, Shuji Higashi, Yutaka Nagai, Ryotaro Ozasa, Shinya Fujita, Takashi Yokoi, Shosaku Nomura, Department of Cardiovascular Internal Medicine, Suzuran Hospital, Kobe, Japan, and others)
Abstract: We measured and compared the elevated levels of platelet-derived microparticles (PDMP) and endothelial cell-derived microparticles (EDMP) in healthy subjects and patients who underwent percutaneous coronary intervention (PCI). In order to confirm the validity of the standardized PDMP measurement method and to propose a standardized EDMP flow cytometry detection method. Therefore, three standard beads were used in the forward scatter gate setting for the extraction of PDMP and EDMP. MP were detected in plasma samples from healthy subjects (n=29) and PCI patients (n=20). Some PDMP were observed in healthy control subjects; however, the patients had significantly more PDMP than those in controls. While EDMP were rarely observed in healthy control subjects, significantly more EDMP were observed in patients than those in controls. The numbers of CD144-positive and CD142-positive MPs were very low in healthy control subjects. In contrast, the numbers of CD142- and CD144-positive MPs were relatively high in the patients, and we also found a positive correlation between the numbers of each cell type (R2=0.719). These results suggest that 1) CD142 expression is induced on the surface of damaged or activated endothelial cells, 2) both MP expressing
CD142 and MP expressing CD144 are released from endothelium cells, and 3) MP expressing CD142 might affect thrombus formation in patients that have undergone PCI.
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