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01.Pre- and Post-Harvest Intervention Strategies for Controlling Salmonella Contamination in Broiler Production (pp. 1-38)
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Alternative Methods for Rapid Whole-Cell Detection of Salmonella spp (pp. 135-154) $100.00
Authors:  (Bledar Bisha, Lawrence D. Goodridge, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado, USA)
Abstract:
Culture-based methods for detection of Salmonella spp. in food demand the performance of several steps in stages (preenrichment, enrichment, selective plating, identification, and confirmation). This results in significant time-to-detection, media and reagents and labor needed. As a consequence of the need for rapid and specific detection techniques, molecular-based rapid detection methods such as real time polymerase chain reaction (RT-PCR), nucleic acid sequence based amplification (NASBA) or immunoassays have been developed. These methods, however, may still be negatively affected by interference from inhibitors, may be qualitative in nature or require extraction of target molecules (DNA, rRNA, mRNA) prior to analysis allowing for possible contamination or amplification of target sequences from non-viable cells. Alternative rapid detection techniques for Salmonella spp. in food have been developed which accomplish identification and enumeration of whole-cell targets. Fluorescence in situ hybridization (FISH) employs sequence-specific rRNA-targeted fluorescently-labeled oligonucleotide probes to specifically hybridize whole permeabilized bacterial cells. FISH can be coupled with fluorescence microscopy, flow cytometry or capillary electrophoresis to achieve detection and enumeration. Bacteriophage-based detection techniques for Salmonella spp. which make use of the inherent specificity of phages for their target bacterial host have also been described. In addition, biosensor-based detection may offer simplicity and high throughput capability for whole-cell detection of Salmonella spp. in food. These alternative methods for whole-cell detection of Salmonella spp. have shown potential as sensitive, specific, and quantitative methods for detection and identification of this pathogen in foods. The advantages and disadvantages of these methods as well as the current status of development and future directions are discussed here. 


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Alternative Methods for Rapid Whole-Cell Detection of Salmonella spp (pp. 135-154)