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Earthworms for Monitoring Metal Contamination
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Authors: Barbara Plytycz, Joanna Homa, Azwady nor Aziz, László Molnár, Peter Kille, A. John Morgan (Jagiellonian Univ., Krakow, Poland, Cardiff Univ) 
Book Description:
Earthworms are widely used to assess the bioavailability and deleterious effects of metals in contaminated soils. This book discusses some of the direct and indirect methods that have recently been used to determine the ligand-binding speciation and the extent of the toxicologically-important bio-reactive metal and metalloid fractions in the cells of earthworms as receptor organisms. It proceeds to describe suites of cell-based biomarkers (e.g. morphometrics; neutral red retention time and immuno-competence assays), and their variants, that are more-or-less widely used for reporting chemically-evoked stress in earthworms under laboratory, semi-field, and field exposure conditions. NMR-based metabonomic profiling of the tissues of stressed earthworms is reviewed, and the potential of infra-red microspectroscopy for determining the biochemical profiles of specific cells in different functional states is highlighted. The way that the rapidly expanding genomic database is beginning to inform and propel research designed to further understanding of the fundamental mechanisms underpinning metal trafficking and toxicosis at the level of cells, partly through the provision of immuno-histochemical and in situ hybridization ‘tools’, is discussed. The book concludes with the prediction that the future will see the adoption of high-throughput cellular, as well as molecular-genetic, biomarker techniques in earthworm ecotoxicology, possibly with parallel metal fractionation measurements done as a desirable component of experimental design.

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Table of Contents:
Preface
1. Introduction, pp. 1-4
2. Cellular Biomarkers: General, pp. 5-6
3. Earthworm Chloragocytes: Metal Relationships, pp. 7-11
4. Earthworm Chloragocytes: Morphological Plasticity, pp. 13-22
5. Chloragocytes: Metabonomics, pp. 23-24
6. Coelomocytes: Morphology and Composition, pp. 25-30
7. Coelomocytes: Immunocompetence and Soil Quality, pp. 31-47
8. Earthworm Genomics, pp. 49-52
9. Conclusion, pp. 53-76
Index

   Binding: Softcover
   Pub. Date: 2011
   ISBN: 978-1-60692-380-1
   Status: AV
  
Status Code Description
AN Announcing
FM Formatting
PP Page Proofs
FP Final Production
EP Editorial Production
PR At Prepress
AP At Press
AV Available
  
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Earthworms for Monitoring Metal Contamination