Abstract: Several nanomaterials have been developed in recent years. Their properties are unique and qualitatively different from those of larger than submicron size. Nanomaterials offer real and potential applications in modern technology, science and medicine i. e. in advanced materials, electronics, biomedicine, pharmaceuticals, cosmetics, food production, environmental detection and monitoring. Nanomaterials can be either natural or manmade. The first are favored over synthetic polymer based substances. Starch as a nontoxic, cheap and renewable biopolymer is particularly suitable for the development of environment friendly, biocompatible, and functional nanoparticles. Several physicochemical methods are used for preparation of starch derived nanoparticles. Some of them use various options of starch granule disintegration in waterin- oil emulsions and cross-linking. P rocessing of granular, pregelatinised or hydrolysed biopolymer using a mechanical treatment, such as extrusion at elevated temperature under conditions of high shear and simultaneous cross-linking are the other routes. Acid or enzymatic hydrolysis of starch followed by ball milling or membrane dialysis of aqueous dimethyl sulfoxide solution of starch against absolute ethanol was also reported. Aforementioned methods are laborious and energy consuming. They produced starch particles ranging in size from several m to 300 nm. Because of chemicals used, such products could not be utilized in food applications. Recently, an alternative way of preparation of starch originated nanoparticles was proposed. In this procedure, starch granules were subjected to severe drying and/or multiple deep-freezing/thawing cycles followed by mechanical crushing in absolute ethanol suspension. The major fraction of the resulting mixtures was made up by the particles of the size in the range of 100-300 nm. They were accompanied by the 50-100 and below 50 nm particles (about 10% and 5%, respectively). Polysaccharide nanoparticles readily agglomerate and swell on contact with cold water, which caused difficulties in dispersing them in this solvent. However, they could be easily dispersed in both polar and non-polar organic solvents. The submicron size of polysaccharide nanoparticles offers a number of distinct advantages over native starch granules. One of importance is the opportunity to obtain suspensions and emulsions with unique colloidal and rheological properties, better availability and dispersion of nutrients or food additives, as well as better sorption ability of the dry particles and their susceptibility for enzyme hydrolysis and chemical reactions. Their use as thickeners or rheology modifiers (in foods, paints, inks), adhesive or adhesive additives, matrix material or fillers in coating applications and biodegradable polymers, drug delivery agents in pharmacy, selective binders and removers of chemicals and pathogens, carriers of colorants, flavors and fragrances in cosmetics and foods, for delivery of pesticides, fertilizers and chemicals in agriculture, in paper-making and packaging industry (adhesive, paper and cardboard surface treatment, gloss etc.) is among potential applications of starch nanoparticles. For their barrier properties starch originated nanoparticles could be used as components of packaging foils or films preventing products from rotting.